Vitrification in Assisted Reproduction: A User's Manual and Trouble-Shooting Guide (Reproductive Medicine and Assisted Reproductive Techniques)
By Michael J. Tucker, Juergen Liebermann
Publisher: Informa Healthcare
Number Of Pages: 304
Publication Date: 2007-11-26
ISBN-10 / ASIN: 0415408822
ISBN-13 / EAN: 9780415408820
Binding: Hardcover
After 20 years of the first publication on vitrification as an ice crystal-free cryopreservation method for embryos, there is a chronic need for a book that addresses this cryopreservation technique in detail, outlining those techniques that result in success, and providing recommended means of overcoming the problems typically encountered. The book will supply a historical overview of the principles and development of vitrification techniques in animals and humans; the various consumables utilized for the vitrification of cells and tissue and suppliers thereof; and the vitrification and warming technique for oocytes, sperm, pronuclear stage embryos, cleavage stage embryos, blastocysts, ovarian tissue, and embryonic stem cells. Wherever applicable, the set-up including protocol, operation, maintenance, and trouble-shooting of the different protocols used for vitrifying various stages of cells and tissues will also be covered.
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